The Ark Theme played several times in Raiders of the Lost Ark and briefly appeared in Indiana Jones and the Last Crusade, and Indiana Jones and the Kingdom of the Crystal Skull. It is a haunting melody, most prominently consisting of three downward notes. The music is first heard when the men from Washington discuss the Ark with Indiana, and it reaches an apex when the Ark is opened, and the Power of God rains down upon the Nazis.
The motif is heard in Last Crusade once, when Indy and Elsa are exploring the Venetian catacombs and see a carving of the relic. It is also heard in Kingdom of the Crystal Skull twice, both times within Hangar 51; the government's hiding place of the lost Ark, as seen at the end of the first film.